STEMCELL Technologies ImmunoCult ImmunoCult Mouse B Cell Expansion Kit
- 研究用
ImmunoCult™ Mouse B Cell Expansion Kit（製品コード：ST-100-1003）は、煩雑なフィーダー細胞を使用せずに、無血清条件下でマウスのB細胞を着実に拡大培養するキットです。
本品は、マウスの脾臓細胞から高収量のB細胞を増殖する、最適な培養条件を提供します。ImmunoCult™-XF B Cell Base Medium および ImmunoCult™ ACF Mouse B Cell Expansion Supplement から構成されており、使い勝手の良い堅牢な培養系です。わずか6～9日間の培養後に細胞を回収し、下流のアプリケーションにそのまま使用できます。
STEMCELL Technologies社の他製品への適合性があります。たとえば、B細胞をEasySep™ Mouse Pan-B Cell Isolation Kit（ST-19844）で分離した後、本品ですぐに増殖できます。
2018/05/14 12:00の製品情報
本製品は研究目的にのみ使用し、人や動物の医療用・臨床診断用・食品用としては使用しないようにご注意ください。
製品の特長
ImmunoCult™ Mouse B Cell Expansion Kitで、マウスB細胞を効率よく増殖！
- 血清・フィーダー細胞・専用プレートなしで、マウスB細胞をin vitro増殖
- 最適化済みの使いやすい培養系で、ワークフローを簡略化
- 拡大培養したマウスB細胞を、一貫した収量で取得
B細胞培養の流れ
Figure 1. Protocol Diagram for Culturing Mouse B Cells with ImmunoCult™ Mouse B Cell Expansion Kit
B cells isolated from mouse spleen using EasySep™ Mouse Pan-B Cell Isolation Kit were cultured in complete Mouse B Cell Expansion Medium as described in Directions For Use (steps 1 - 7). B Cells were harvested on day 9 for analysis. Cells can also be harvested at earlier time points depending on different applications.
データ紹介
マウスB細胞の増殖
Figure 2. Expansion of Mouse B Cells with ImmunoCult™ Mouse B Cell Expansion Kit
B cells isolated from mouse spleen using EasySep™ Mouse Pan-B Cell Isolation Kit were cultured as described in Figure 1. Fold expansion of viable cells is shown with bar graphs representing the mean ± SEM (n = 8). B cells expanded 176.9 ± 29.8-fold after 9 days of culture.
マウスB細胞の成熟
Figure 3. Maturation of Mouse B Cells with ImmunoCult™ Mouse B Cell Expansion Kit
B cells isolated from mouse spleen using EasySep™ Mouse Pan-B Cell Isolation Kit were cultured as described in Figure 1. A) Expression of B220 and CD138 and B) Expression of TACI (CD267) and CD86 were analyzed by flow cytometry at each timepoint (data represents mean ± SEM, n = 8). An increase in CD86 cell surface expression indicates B cell activation; a decrease in B220 and an increase in CD138 and TACI cell surface expression indicates maturation of B cells to plasmablasts or plasma cells.
マウスB細胞の形態
Figure 4. Robust Growth of Mouse B Cells when Cultured with ImmunoCult™ Mouse B Cell Expansion Kit
B cells isolated from mouse spleen using EasySep™ Mouse Pan-B Cell Isolation Kit were cultured as described in Figure 1. Cells were imaged at A) 10X magnification on day 0, B) 10X magnification on day 3, C) 40X magnification on day 6, and D) 40X magnification on day 9.
