STEMCELL Technologies EpiCult EpiCult-B Medium Kit (Mouse)
- 研究用
フィーダーを使用したマウス乳腺コロニーアッセイ(Mammary colony-forming unit/Ma-CFU assay)に最適化された無血清培地です。
管腔と筋上皮の乳腺上皮前駆細胞の増殖と検出をサポートします。
製品の特長
- フィーダーを使用した乳腺コロニーアッセイ(Mammary colony-forming unit/Ma-CFU assay)に最適化された無血清培地
- 管腔と筋上皮の乳腺上皮前駆細胞の増殖と検出をサポート
- ウシ下垂体抽出物を含まず
- 分化の極性を検出する3D形態形成アッセイ(3D Matrigel-based assay)に使用可能
乳腺上皮前駆細胞の分離・同定のワークフロー
Figure 1. Protocol for Isolation and Identification of Human and Mouse Mammary Epithelial Progenitor Cells
Phase contrast photographs of (A) a pure human myoepithelial cell colony, (B) a pure human luminal cell colony, and (C) a mixed human colony. (D) is a mouse colony. Unlike human mammary CFC colonies, subtypes of mouse mammary epithelial cell colonies are not easily identifiable. All colonies were cultured in either EpiCult™-B (Human: Catalog #05601) or EpiCult™-B (Mouse:Catalog #5610) in the presence of an irradiated NIH 3T3 feeder layer. Colonies were visualized by staining with Wright"s Giemsa. (E) is a picture of mammospheres obtained from primary human mammary epithelial cells and (F) is an image of tumorspheres obtained from MCF7 human breast cancer cell line.