STEMCELL Technologies mTeSR FreSR-S
- 研究用
FreSR™-S(ST-05859)は、ヒトES/iPS細胞をシングルセルとして凍結保存するための、動物性成分を含まない(animal component-free)、定義された組成(defined)の凍結保存培地です。
本品はすぐに使用可能な完全培地であり、mTeSR™1、mTeSR™ Plus、TeSR™2、またはTeSR™-E8™で培養したヒト多能性幹細胞(hPSC)に推奨されます。
凍結保存したhPSCは、-135°C(液体窒素)またはそれ以下で保存して下さい。
製品の特長
FreSR™-Sをもちいて、ヒト多能性幹細胞のシングルセルを動物性成分フリー条件下で凍結保存できます
保存した細胞は高い生存率と回収率を示します
![05859fresr-s_fig1[1].jpg](/products/images/05859fresr-s_fig1%5B1%5D.jpg)
hPSCs cryopreserved as single cells using FreSR™-S have (A) higher post-thaw recovery (number of cells recovered / number of cells frozen) and (B) maintain higher viability (number of live cells / total number of cells) compared to competitor medium. All data values are plotted as percentages, (n=18, p < 0.0001 for each).
より多くのバイアル数を保存できます
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hPSCs are cryopreserved in FreSR™-S at lower cell density compared to traditional methods. Graph indicates the number of vials cryopreserved for each well of a 6 well plate that is harvested.
Note: 1 vial is typically thawed and seeded directly into 1 well of a six well plate. Cells should be cultured as aggregates after thawing.
凍結融解後の細胞は正常な核型を示します
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Karyograms of (A-B) WLS-4D1 hiPS cells and (C-D) H1 hES cells that were frozen and thawed as single cells using FreSR™-S. (A, C) Thawed cells were seeded into culture containing TeSR™-E8™ medium and 10 µM Y-27632 and maintained as aggregates for five passages. (B,D) hPSCs were also subjected to a second freeze-thaw cycle as single cells with FreSR™-S and cultured for five passages as aggregates prior to collection of karyotype data.