STEMCELL Technologies EasySep EasySep Serology Whole Blood CD3 Positive Selection Kit
- 研究用
EasySep™ Serology Whole Blood CD3 Positive Selection Kit(ST-18981)は、新鮮ヒト全血から高純度のCD3陽性細胞を免疫磁気ポジティブセレクションで単離するためにデザインされています。本キットはヒトCD3+細胞を、CD3表面マーカーを認識する抗体によるポジティブ分離のターゲットとします。目的の細胞は抗体と磁性粒子でラベルされ、EasySep™ magnetによりカラムを使用せずに分離されます。不要な細胞はシンプルに溶液を流し出す事で除去され、目的の細胞はチューブ内に留まります。分離した細胞はHLA血清学、フローサイトメトリー、または核酸(DNA/RNA)抽出といった下流のアプリケーションにすぐご利用いただけます。
製品の特長
EasySep™ Serology Whole Blood CD3 Positive Selection Kitをもちいて、ヒト全血から高純度のT細胞をポジティブ分離!
Starting with human whole blood, the CD3+ cell content of the isolated fraction typically ranges from 96.7 - 99.6% (note: as the CD3 antibody used for flow cytometry staining was fully blocked by the CD3 antibody used to isolate the cells, staining of T cells was assessed by labeling with anti-CD2; gated on CD45+). In the above example, the purities of the start and final isolated fractions are 25.4% and 98.7% (gated on CD45+), respectively.
NOTE: Red blood cells were removed by lysis prior to flow cytometry.
NOTE: The magnetic particles present on the surface of the isolated CD3+ cells will cause a large FSC/SSC shift when the samples are run on a flow cytometer. This will make it difficult to gate on a specific lymphocyte population based on FSC/SSC. Instead, we recommend first gating the cells on CD45 and examining the lymphocyte populations based on the CD45+ gate.