STEMCELL Technologies STEMdiff STEMdiff Cardiomyocyte Plating Kit
- 研究用
- 新製品
STEMdiff™ Cardiomyocyte Plating Kit (ST-100-1121) は、ヒト多能性幹細胞 (hPSC) 由来心筋細胞のプレーティング効率を向上させ、細胞損失を最小限に抑えます。STEMdiff™ Cardiomyocyte Plating Supplement と STEMdiff™ Cardiomyocyte Support Medium で構成されており、解離、解凍、再播種後のhPSC由来心筋細胞の収量を倍増させることができます。
本品は、STEMdiff™ Ventricular Cardiomyocyte Differentiation Kit または STEMdiff™ Atrial Cardiomyocyte Differentiation Kit を用いて作製したhPSC由来心筋細胞用に設計されています。心筋細胞の播種効率を2倍にし、実験のスループットを向上させることができます。
本品は、hPSC由来心筋細胞に最適化された完全ワークフローの一部であり、以下のSTEMdiff™ cardiomyocyte製品群と適合性があります。
STEMdiff™ Ventricular Cardiomyocyte Differentiation Kit
STEMdiff™ Atrial Cardiomyocyte Differentiation Kit
STEMdiff™ Cardiomyocyte Dissociation Kit
STEMdiff™ Cardiomyocyte Support Medium
STEMdiff™ Cardiomyocyte Freezing Medium
STEMdiff™ Cardiomyocyte Maintenance Kit
製品の特長
STEMdiff™ Cardiomyocyte Plating Kitをもちいて、hPSC由来心筋細胞のプレーティング効率を向上させることができます
- hPSC由来心筋細胞の播種効率を上げ、得られる細胞数を倍増させます
- 再播種後のhPSC由来心筋細胞は、安定した電気的プロファイルを維持します
- 融解および解離後の心筋細胞をより効率よく再播種でき、時間的にも経済的にも節約できます
データ紹介
播種効率の向上
Plating efficiency of hPSC-derived cardiomyocytes was assessed 1 day after replating at low density in either STEMdiff™ Cardiomyocyte Support Medium or STEMdiff™ Cardiomyocyte Plating Medium. Across 4 hPSC lines, plating efficiency was improved when cardiomyocytes were replated in STEMdiff™ Cardiomyocyte Plating Medium (51% ± 3%, n=15) compared to STEMdiff™ Cardiomyocyte Support Medium (16% ± 1%, n=15).
再播種後のhPSC由来心筋細胞の形態
Representative images of replated hPSC-derived cardiomyocytes. hPSC-derived cardiomyocytes were replated at low density in either STEMdiff™ Cardiomyocyte Support Medium or STEMdiff™ Cardiomyocyte Plating Medium. 1 day after replating, hPSC-derived cardiomyocytes exhibit improved attachment when replated with STEMdiff™ Cardiomyocyte Plating Medium.
再播種後のhPSC由来心筋細胞の安定した拍動特性
Microelectrode array recordings of hPSC-derived cardiomyocytes replated using either STEMdiff™ Cardiomyocyte Support Medium or STEMdiff™ Cardiomyocyte Plating Medium. Cardiomyocytes were maintained for 1 week with STEMdiff™ Cardiomyocyte Maintenance Medium. Across 4 hPSC lines, cardiomyocytes retain a stable beat rate when replated using STEMdiff™ Cardiomyocyte Plating Medium.
