STEMCELL Technologies StemSpan StemSpan Erythroid Expansion Supplement
- 研究用
StemSpan™ Erythroid Expansion Supplement（100X）は、ヒトの臍帯血（CB）または骨髄（BM）から分離したCD34+細胞から、赤血球前駆細胞を選択的に増殖、分化させる培養用サプリメントです。組み換えヒトサイトカイン（SCF、IL-3、EPO）と合成成分を配合した100倍濃縮液です。
無血清培地に添加すると、CD34+ヒトCB細胞の液体培養では通常、CD34+細胞1個のインプットあたり14日間で数千個の赤血球細胞を産生させます。
本品は、以下のStemSpan™培地との併用が意図されています。
・ StemSpan™ SFEM (ST-09600)
・ StemSpan™ SFEM II (ST-09605)
・ StemSpan™-XF (ST-100-0073)
・ StemSpan™-ACF Erythroid Expansion Medium (ST-09860)
2018/05/14 12:00の製品情報
本製品は研究目的にのみ使用し、人や動物の医療用・臨床診断用・食品用としては使用しないようにご注意ください。
製品の特長
StemSpan™ Erythroid Expansion Supplementで、ヒト赤血球細胞を無血清で増殖
- 臍帯血または骨髄のCD34+細胞から、液体培養で赤血球細胞を大量に産生
- 各種のStemSpan™無血清培地への添加に最適
（StemSpan™ SFEM IIと併用すれば、臍帯血CD34+細胞から赤血球細胞を他の市販無血清培地比で4倍まで増殖可能） - 100倍濃縮液として提供
（解凍、混合後に任意の培地に加えるだけ）
データ紹介
Erythroid Expansion Supplementを添加したStemSpan™ SFEM培地をもちいて、ヒト臍帯血CD34陽性細胞の増殖と細胞系列特異的分化により産生した赤芽球
Flow cytometry dot plots showing expression of the HSPC marker CD34 and erythroid markers CD71 and glycophorin-A (GlyA) (A) before culture and (B,C) after 14 days of culture of enriched CD34+ CB cells in StemSpan™ SFEM containing Erythroid Expansion Supplement. The frequency of CD34+ cells declined from ~60% before culture to <0.1% after 14 days. In parallel, erythroid cells gradually accumulated from levels of <1% before culture to >90% by day 14. The bulk of cell population recovered from 14-day culture consisted of CD71+GlyA+ erythroblasts. More immature CD71+GlyA- progenitors and proerythroblasts, as well as more differentiated CD71-/low GlyA+ normoblasts were also present at low frequencies.
Erythroid Expansion Supplementを添加した各種StemSpan培地をもちいて、ヒト臍帯血CD34陽性細胞から産生した赤血球細胞
(A) Average numbers of erythroid cells generated after culturing purified CD34+ CB cells (n = 5) for 14 days in StemSpan™ SFEM (black bars), SFEM II (grey bars), or StemSpan™-ACF Erythroid Expansion Medium (ACF-E, orange bars) media containing StemSpan™ Erythroid Expansion Supplement (Catalog #02692). Shown are the number of erythroid cells that express CD71 and/or Glycophorin A (GlyA) per input CD34+ cell. (B) The percentages of the different erythroid cell subsets generated in these cultures are shown, including CD71+GlyA+ erythroblasts, immature CD71+GlyA- erythroid progenitor cells and pro-erythroblasts, and CD71-/lowGlyA+ normoblasts. All three media supported the generation of thousands of erythroid cells per CB-derived CD34+ cell plated.
Erythroid Expansion Supplementを添加した各種StemSpan培地をもちいて、ヒト臍帯血CD34陽性細胞から産生した赤血球細胞
Yields and percentages of erythroid cells produced after culturing purified CD34+ CB cells (n = 5) for 14 days in StemSpan™ SFEM, SFEM II or ACF-E media containing Erythroid Expansion Supplement. Erythroid cells were identified by flow cytometry after staining with antibodies against CD71 and GlyA. The % erythroid cells represent the percentage of cells that express CD71 and/or GlyA. *CI: Confidence Interval.
