STEMCELL Technologies StemSpan StemSpan Erythroid Expansion Supplement
- 研究用
StemSpan™ Erythroid Expansion Supplement(100X)は、ヒト臍帯血(CB)または骨髄(BM)から分離したCD34+細胞から赤血球前駆細胞を選択的に増殖、分化させるよう配合した組み換えヒトサイトカイン(SCF、IL-3、およびEPO)と合成成分を含んでいます。無血清培地に添加すると、通常CD34+ヒトCB細胞の14日間の液体培養において、CD34+細胞1個あたり数千個の赤血球細胞を産生させます。
StemSpan™ Erythroid Expansion Supplement (100X)は以下のStemSpan™培地と組み合わせた使用を想定しています。
・ StemSpan™ SFEM (ST-09600)
・ StemSpan™ SFEM II (ST-09605)
・ StemSpan™-XF (ST-100-0073)
・ StemSpan™-ACF Erythroid Expansion Medium (ST-09860)
製品の特長
ヒト赤血球細胞を無血清で増殖させるためのサプリメントです
- CD34+臍帯血または骨髄細胞から開始する液体培養において、赤血球細胞を大量に取得できます
- StemSpan™培地との使用に最適化されています
(StemSpan™ SFEM IIとの併用時、臍帯血CD34+細胞から赤血球細胞を他の市販無血清培地比で4倍まで増殖可能) - 100倍濃縮液として提供されます
(解凍、混合後に任意の培地に直接添加可能)
データ紹介
Erythroid Expansion Supplementを添加したStemSpan™ SFEM培地をもちいて、ヒト臍帯血CD34陽性細胞の増殖と細胞系列特異的分化により産生した赤芽球
![Flow cytometry dot plots showing expression of CD34, CD71, and GlyA before and after culture in StemSpan™](https://www.stemcell.com/media/images/data-figures/02692/28084-fig1.jpg)
Flow cytometry dot plots showing expression of the HSPC marker CD34 and erythroid markers CD71 and glycophorin-A (GlyA) (A) before culture and (B,C) after 14 days of culture of enriched CD34+ CB cells in StemSpan™ SFEM containing Erythroid Expansion Supplement. The frequency of CD34+ cells declined from ~60% before culture to <0.1% after 14 days. In parallel, erythroid cells gradually accumulated from levels of <1% before culture to >90% by day 14. The bulk of cell population recovered from 14-day culture consisted of CD71+GlyA+ erythroblasts. More immature CD71+GlyA- progenitors and proerythroblasts, as well as more differentiated CD71-/low GlyA+ normoblasts were also present at low frequencies.
Erythroid Expansion Supplementを添加した各種StemSpan培地をもちいて、ヒト臍帯血CD34陽性細胞の培養により産生した赤血球細胞
![02692-Fig2.jpg](/products/images/02692-Fig2.jpg)
(A) Average numbers of erythroid cells generated after culturing purified CD34+ CB cells (n = 5) for 14 days in StemSpan™ SFEM (black bars), SFEM II (grey bars), or StemSpan™-ACF Erythroid Expansion Medium (ACF-E, orange bars) media containing StemSpan™ Erythroid Expansion Supplement (Catalog #02692). Shown are the number of erythroid cells that express CD71 and/or Glycophorin A (GlyA) per input CD34+ cell. (B) The percentages of the different erythroid cell subsets generated in these cultures are shown, including CD71+GlyA+ erythroblasts, immature CD71+GlyA- erythroid progenitor cells and pro-erythroblasts, and CD71-/lowGlyA+ normoblasts. All three media supported the generation of thousands of erythroid cells per CB-derived CD34+ cell plated.
Erythroid Expansion Supplementを添加した各種StemSpan培地をもちいて、ヒト臍帯血CD34陽性細胞の培養により産生した赤血球細胞
![02692-Table1.jpg](/products/images/02692-Table1.jpg)
Yields and percentages of erythroid cells produced after culturing purified CD34+ CB cells (n = 5) for 14 days in StemSpan™ SFEM, SFEM II or ACF-E media containing Erythroid Expansion Supplement. Erythroid cells were identified by flow cytometry after staining with antibodies against CD71 and GlyA. The % erythroid cells represent the percentage of cells that express CD71 and/or GlyA. *CI: Confidence Interval.