STEMCELL Technologies MesenCult MesenCult Osteo Stimulatory Kit (Mouse)
- 研究用
MesenCult™ Osteogenic Stimulatory Kit (Mouse) (ST-05504) は、マウス緻密骨や骨髄由来の間葉系幹細胞および前駆細胞 (MSC)、ならびにマウス胚性線維芽細胞 (MEF) をin vitroで骨芽細胞へ分化させる培地です。
本品は、マウスMSCおよびMEFの骨形成を強力に誘導します。このことは、骨の分化と成熟に関与する重要な転写産物のqPCR解析、およびアリザリンレッド染色によって証明されています。本品は、MSCとMEFの特性評価、および骨発生の研究に推奨されます。
製品の特長
MesenCult Osteogenic Stimulatory Kitで、マウスMSCおよびMEFを骨芽細胞に分化
Figure 1. Differentiation of mouse compact bone-derived mesenchymal stem cells (CB-MSCs) with the MesenCult™ Osteogenic Stimulatory Kit (Mouse)
Compact bone-derived MSCs exhibited superior expression of key osteogenic transcripts involved in bone differentiation and maturation following treatment with MesenCult™ Osteogenic Stimulatory Kit (Mouse)*
Figure 2. Compact bone-derived MSCs display robust bone matrix deposition following treatment with MesenCult™ Osteogenic Stimulatory Kit (Mouse) as demonstrated by Alizarin Red-staining
Ca2+ deposits are detected with Alizarin Red.
For both the qPCR analysis and Alizarin-Red staining assay, the mouse compact bone-derived MSCs were cultured in vitro for three passages, purified with EasySep™ Mesenchymal Stem/Progenitor Cell Enrichment Kit (Mouse, Catalog #19771), and then cultured for another passage, prior to differentiation with the MesenCult™ Osteogenic Stimulatory Kit (Mouse).
関連リンク
間葉系幹細胞(MSC)の分離・培養・分化 【E-Learning】