STEMCELL Technologies MesenCult MesenCult Adipogenic Differentiation Kit (Mouse)
- 研究用
MesenCult™ Adipogenic Differentiation Kit (Mouse) (ST-05507)は、マウス間葉系幹細胞/前駆細胞(MSC)、脂肪組織由来MSC(ADSC)、およびマウス胚性線維芽細胞(MEF)を、脂肪生成系列の細胞にin vitroで分化させるために特化した組成の培地です。
注: MesenCult™ Adipogenic Differentiation Mediumには、L-Glutamine(製品コード:ST-07100)を添加する必要があります。
製品の特長
MesenCult Adipogenic Differentiation Mediumをもちいて、マウスMSC、ADSC、およびMEFを脂肪細胞へ分化させることができます
Mouse BM-Derived MSCs Cultured in the MesenCult™ Expansion Kit (Mouse) Maintain Multi-Lineage Differentiation Potential
Mouse BM-derived MSCs derived and cultured in MesenCult™ Expansion Medium (Mouse) with MesenPure™ were differentiated into cells of (A) adipogenic and (B) osteogenic lineages when cultured under hypoxic conditions using the MesenCult™ Adipogenic Differentiation Kit (Mouse; Catalog #05507) and MesenCult™ Osteogenic Stimulatory Kit (Mouse; Catalog #05504), respectively. Adipocytes were stained with Oil Red O for lipids, and osteoblasts were stained with silver nitrate (von Kossa) for calcification and for alkaline phosphatase activity. Compact bone- and adipose tissue-derived MSCs as well as MEFs cultured in complete MesenCult™ Expansion Medium (with or without MesenPure™) were also successfully differentiated into adipocytes and osteoblasts (data not shown).