ClonaCellでできること

• 従来の限界希釈クローニングの課題を克服する、実証済みの半固形クローニング法で時間とリソースを節約
• マウス、ラット、ヒト、ウサギ、ハムスターの細胞から作製されたハイブリドーマの育成と保存
• CHO細胞株作製におけるサブクローニング効率の向上

ClonaCellが使用される理由

• 簡便　半固形クローニングで時短。空のクローニングウェルによる資源の無駄を回避
• 実証済み　CHO、ハイブリドーマ、ミエローマなどで優れたクローニング効率と増殖
• 汎用性　メチルセルロース基礎培地は、細胞の種類に合わせて簡単にカスタマイズ可能。組成、梱包、サイズのカスタマイズに応相談
• 一貫性　ロット間の再現性を確実にする厳しい性能試験を実施
• 信頼性　業界で認められた製造の専門知識と、比類のない技術サポート

動物性成分フリーの液体培地は、CHO細胞の適応、増殖およびクローニングに利用できます。

限界希釈法 vs 半固形クローニング法

従来の限界希釈クローニングに比べて、半固形クローニングでは抗体作製過程を大幅に短縮することができます。

詳しくはこちらを御覧ください

ClonaCell関連　動画マニュアル・ウェビナー

ClonaCellを用いた半固形クローニングの利点

ClonaCellのメリットを解説した動画です

用途別　おすすめClonaCell製品

ハイブリドーマ作製向け

CHOとその他の細胞株作製向け

STEMCELL Technologies社の細胞株作製用製品のラインナップはこちら＞＞

ClonaCell各製品の詳細

ハイブリドーマ作製向け

略語：DHFR = Dihydrofolate reductase、GS = glutamine synthetase

ClonaCell™-HY Hybridoma Kitの内容物

CHO細胞とその他の細胞株作製向け

略語：DHFR = Dihydrofolate reductase、GS = glutamine synthetase

ClonaCellの主要文献　アプリケーション別

抗コロナウイルス抗体作製（ハイブリドーマ作製）

• Berry JD et al. (2004) Development and characterisation of neutralising monoclonal antibody to the SARS-coronavirus. J Virol Methods 120(1): 87–96.
• Nieto-Torres JL et al. (2011) Subcellular location and topology of severe acute respiratory syndrome coronavirus envelope protein. Virology 415(2): 69–82.
• Lip KM et al. (2006)Monoclonal antibodies targeting the HR2 domain and the region immediately upstream of the HR2 of the S protein neutralize in vitro infection of severe acute respiratory syndrome coronavirus. J Virol 80(2) 941-50. J Virol 90(9) 4357–4368.

細胞株作製

• Ling SSM et al. (2015) Instrumental Role of Helicobacter Pylori γ-Glutamyl Transpeptidase in VacA-Dependent Vacuolation in Gastric Epithelial Cells. PLoS One 10(6): e0131460.
• Rodríguez L et al. (2015) Generation of Monoclonal Antibodies Specific of The Postfusion Conformation of The Pneumovirinae Fusion (F) Protein. J Virol Methods.
• Young J et al. (2015) A Novel Immunoassay to Measure Total Serum Lymphotoxin-α Levels in The Presence of An Anti-LTα Therapeutic Antibody. J Immunol Methods Epub ahead.
• Chronopoulou E et al. (2014) Hybridoma Technology for The Generation of Rodent mAbs via Classical Fusion. In: Ossipow V, N Fischer (Eds.) Monoclonal Antibodies: Methods and Protocols, Methods in Molecular Biology. 2nd Ed. New York: Springer Science+Business Media.: pp47–70.
• Date Y et al. (2014) Label-Free Impedimetric Immunoassay for Trace Levels of Polychlorinated Biphenyls in Insulating Oil. Anal Chem 86(6): 2989–2996.
• García-Barreno B et al. (2014) Characterization of An Enhanced Antigenic Change in The Pandemic 2009 H1N1 Influenza Virus Haemagglutinin. J Gen Virol 95(PART 5): 1033–1042.
• Tan GS et al. (2014) Characterization of a Broadly Neutralizing Monoclonal Antibody That Targets The Fusion Domain of Group 2 Influenza A Virus Hemagglutinin. J Virol 88(23): 13580–13592.
• Kelly-Cirino CD & Mantis NJ. (2009) Neutralizing Monoclonal Antibodies Directed Against Defined Linear Epitopes on Domain 4 of Anthrax Protective Antigen. Infect Immun 77(11): 4859–4867.
• Weidanz J a et al. (2006) Levels of Specific Peptide-HLA Class I Complex Predicts Tumor Cell Susceptibility to CTL Killing. J Immunol 177(8): 5088–5097

異なる動物種でのハイブリドーマ作製

• Chronopoulou E et al. (2014) Hybridoma Technology for the Generation of Rodent mAbs via Classical Fusion. In: Ossipow V, N Fischer (Eds.) Monoclonal Antibodies: Methods and Protocols, Methods in Molecular Biology. 2nd Ed. New York: Springer Science+Business Media.: pp47–70.
• Cesaro A et al. (2012) An Inflammation Loop Orchestrated by S100A9 and Calprotectin Is Critical for Development of Arthritis. PLoS One 7(9): e45478.
• Smith S a. et al. (2012) Persistence of Circulating Memory B Cell Clones with Potential for Dengue Virus Disease Enhancement for Decades following Infection. J Virol 86(5): 2665–2675.
• Fang L et al. (2008) Essential Role of TNF Receptor Superfamily 25 (TNFRSF25) in the Development of Allergic Lung Inflammation. J Exp Med 205(5): 1037–1048.
• Ulbrandt ND et al. (2006) Isolation and Characterization of Monoclonal Antibodies Which Neutralize Human Metapneumovirus In Vitro and In Vivo. J Virol 80(16): 7799–7806.
• Grimaldi JC et al. (1999) Depletion of Eosinophils in Mice through the Use of Antibodies Specific for C-C Chemokine Receptor 3 (CCR3). J Leukoc Biol 65(6): 846–853.

薬理学と創薬

• Cabral TM et al. (2014) Development of Neutralizing Monoclonal Antibodies against The Pandemic H1N1 Virus (2009) Using Plasmid DNA Immunogen. J Virol Methods 195: 54–62.
• Retamal M et al. (2014) Epitope Mapping of The 2009 Pandemic and the A/Brisbane/59/2007 Seasonal (H1N1) Influenza Virus Haemagglutinins Using mAbs and Escape Mutants. J Gen Virol 95(Pt_11): 2377–2389.
• Hostetter DR et al. (2007) Hip Is A Pro-Survival Substrate of Granzyme B. J Biol Chem 282(38): 27865–27874.
• Jin C et al. (2006) Immunoglobulin G Specifically Binding Plant N-glycans with High Affinity Could Be Generated in Rabbits But Not in Mice. Glycobiology 16(4): 349–357.
• Kuroki M et al. (2005) Preparation of Human IgG and IgM Monoclonal Antibodies for MK-1/Ep-CAM by Using Human Immunoglobulin Gene-Transferred Mouse and Gene Cloning of Their Variable Regions. Anticancer Res 25(6A): 3733–3739.

免疫学研究

• Flyak AI et al. (2015) Mechanism of Human Antibody-Mediated Neutralization of Marburg Virus. Cell 160(5): 893–903.
• Fang L et al. (2008) Essential Role of TNF Receptor Superfamily 25 (TNFRSF25) in The Development of Allergic Lung Inflammation. J Exp Med 205(5): 1037–1048.
• Matsumoto SC et al. (2006) Retinal Dysfunction in Patients with Chronic Chagas’ Disease Is Associated to Anti-Trypanosoma Cruzi Antibodies That Cross-React with Rhodopsin. FASEB J 21: 1–21.

がん研究

• Chen D et al. (2014) Increased Expression of Id1 and Id3 Promotes Tumorigenicity by Enhancing Angiogenesis and Suppressing Apoptosis in Small Cell Lung Cancer. Genes Cancer 5-6(May): 212–225.
• Stern HM et al. (2010) Development of Immunohistochemistry Assays to Assess GALNT14 and FUT3/6 in Clinical Trials of Dulanermin and Drozitumab. Clin Cancer Res 16(5): 1587–1596.
• Chen Y et al. (2007) Armed Antibodies Targeting The Mucin Repeats of The Ovarian Cancer Antigen, MUC16, Are Highly Efficacious in Animal Tumor Models. Cancer Res 67(10): 4924–4932.
• Wittman VP et al. (2006) Antibody Targeting to A Class I MHC-Peptide Epitope Promotes Tumor Cell Death. J Immunol 177(6): 4187–4195.

関連リンク

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  ClonaCell-HY Hybridoma Kit

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  ClonaCell-CHO

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  ClonaCell-FLEX

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  ClonaCell-TCS/G418 Kit

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  ClonaCell-TCS/Hygromycin Kit

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  EasySep Mouse CD19 Positive Selection Kit II

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  EasySep Mouse CD138 Positive Selection Kit

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  EasySep Mouse B Cell Isolation Kit

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  EasySep Mouse Pan-B Cell Isolation Kit